Molecular Methods to Detect and Quantify Botryosphaeriaceae Inocula Associated With Grapevine Dieback in Australia.
Identifieur interne : 000242 ( Main/Exploration ); précédent : 000241; suivant : 000243Molecular Methods to Detect and Quantify Botryosphaeriaceae Inocula Associated With Grapevine Dieback in Australia.
Auteurs : Regina Billones-Baaijens [Australie] ; José Ram N Úrbez-Torres [Canada] ; Meifang Liu [Australie] ; Matthew Ayres [Australie] ; Mark Sosnowski [Australie] ; Sandra Savocchia [Australie]Source :
- Plant disease [ 0191-2917 ] ; 2018.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), ADN fongique (isolement et purification), Amorces ADN (génétique), Ascomycota (classification), Ascomycota (génétique), Ascomycota (physiologie), Australie (MeSH), Maladies des plantes (microbiologie), Reproductibilité des résultats (MeSH), Réaction de polymérisation en chaine en temps réel (méthodes), Spores fongiques (génétique), Spores fongiques (physiologie), Spécificité d'espèce (MeSH), Vitis (croissance et développement), Vitis (microbiologie).
- MESH :
- croissance et développement : Vitis.
- génétique : ADN fongique, Amorces ADN, Ascomycota, Spores fongiques.
- isolement et purification : ADN fongique.
- microbiologie : Maladies des plantes, Vitis.
- méthodes : Réaction de polymérisation en chaine en temps réel.
- physiologie : Ascomycota, Spores fongiques.
- Australie, Reproductibilité des résultats, Spécificité d'espèce.
- Wicri :
- geographic : Australie.
English descriptors
- KwdEn :
- Ascomycota (classification), Ascomycota (genetics), Ascomycota (physiology), Australia (MeSH), DNA Primers (genetics), DNA, Fungal (genetics), DNA, Fungal (isolation & purification), Plant Diseases (microbiology), Real-Time Polymerase Chain Reaction (methods), Reproducibility of Results (MeSH), Species Specificity (MeSH), Spores, Fungal (genetics), Spores, Fungal (physiology), Vitis (growth & development), Vitis (microbiology).
- MESH :
- chemical , genetics : DNA Primers, DNA, Fungal.
- chemical , isolation & purification : DNA, Fungal.
- geographic : Australia.
- classification : Ascomycota.
- genetics : Ascomycota, Spores, Fungal.
- growth & development : Vitis.
- methods : Real-Time Polymerase Chain Reaction.
- microbiology : Plant Diseases, Vitis.
- physiology : Ascomycota, Spores, Fungal.
- Reproducibility of Results, Species Specificity.
Abstract
Botryosphaeria dieback, caused by species of Botryosphaeriaceae, is an important grapevine trunk disease in Australia. Inocula produced by the pathogens are primarily dispersed by rain splash and wind and infect pruning wounds leading to cankers, dieback, and eventually death of vines. The objective of this study was to develop molecular tools to detect and quantify Botryosphaeriaceae inocula from the environment. These tools are essential for investigating spore dispersal patterns of Botryosphaeriaceae pathogens in Australian vineyards. DNA extraction protocols were evaluated and one modified protocol was found suitable for extracting Botryosphaeriaceae DNA from artificially and naturally inoculated Burkard volumetric spore sampler tapes. Multispecies primers and a hydrolysis probe for quantitative PCR (qPCR) were further developed to detect and quantify Botryosphaeriaceae inocula from environmental samples. Specificity tests showed that the multispecies primers were able to amplify the DNA of 10 Botryosphaeriaceae species (58 isolates) found in Australia while none of the 27 nontarget fungal species (90 isolates) tested were amplified. The qPCR assay was suitable for amplifying purified DNA, synthetic DNA fragments (gBlocks), and mixed DNA from spore trap tapes. The qPCR method developed in this study was shown to be rapid and sensitive in detecting Botryosphaeriaceae inocula from the environment using spore traps.
DOI: 10.1094/PDIS-11-17-1854-RE
PubMed: 30673411
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular Methods to Detect and Quantify Botryosphaeriaceae Inocula Associated With Grapevine Dieback in Australia.</title><author><name sortKey="Billones Baaijens, Regina" sort="Billones Baaijens, Regina" uniqKey="Billones Baaijens R" first="Regina" last="Billones-Baaijens">Regina Billones-Baaijens</name><affiliation wicri:level="1"><nlm:affiliation>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author><author><name sortKey="Urbez Torres, Jose Ram N" sort="Urbez Torres, Jose Ram N" uniqKey="Urbez Torres J" first="José Ram N" last="Úrbez-Torres">José Ram N Úrbez-Torres</name><affiliation wicri:level="1"><nlm:affiliation>Summerland Research and Development Centre, Agriculture and Agri-Food Canada, Summerland, BC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Summerland Research and Development Centre, Agriculture and Agri-Food Canada, Summerland, BC</wicri:regionArea><wicri:noRegion>BC</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Meifang" sort="Liu, Meifang" uniqKey="Liu M" first="Meifang" last="Liu">Meifang Liu</name><affiliation wicri:level="1"><nlm:affiliation>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author><author><name sortKey="Ayres, Matthew" sort="Ayres, Matthew" uniqKey="Ayres M" first="Matthew" last="Ayres">Matthew Ayres</name><affiliation wicri:level="1"><nlm:affiliation>South Australian Research and Development Institute, Adelaide, SA, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>South Australian Research and Development Institute, Adelaide, SA</wicri:regionArea><wicri:noRegion>SA</wicri:noRegion></affiliation></author><author><name sortKey="Sosnowski, Mark" sort="Sosnowski, Mark" uniqKey="Sosnowski M" first="Mark" last="Sosnowski">Mark Sosnowski</name><affiliation wicri:level="1"><nlm:affiliation>South Australian Research and Development Institute, Adelaide, SA, Australia; and School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Glen Osmond, SA, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>South Australian Research and Development Institute, Adelaide, SA, Australia; and School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Glen Osmond, SA</wicri:regionArea><wicri:noRegion>SA</wicri:noRegion></affiliation></author><author><name sortKey="Savocchia, Sandra" sort="Savocchia, Sandra" uniqKey="Savocchia S" first="Sandra" last="Savocchia">Sandra Savocchia</name><affiliation wicri:level="1"><nlm:affiliation>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30673411</idno><idno type="pmid">30673411</idno><idno type="doi">10.1094/PDIS-11-17-1854-RE</idno><idno type="wicri:Area/Main/Corpus">000199</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000199</idno><idno type="wicri:Area/Main/Curation">000199</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000199</idno><idno type="wicri:Area/Main/Exploration">000199</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Molecular Methods to Detect and Quantify Botryosphaeriaceae Inocula Associated With Grapevine Dieback in Australia.</title><author><name sortKey="Billones Baaijens, Regina" sort="Billones Baaijens, Regina" uniqKey="Billones Baaijens R" first="Regina" last="Billones-Baaijens">Regina Billones-Baaijens</name><affiliation wicri:level="1"><nlm:affiliation>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author><author><name sortKey="Urbez Torres, Jose Ram N" sort="Urbez Torres, Jose Ram N" uniqKey="Urbez Torres J" first="José Ram N" last="Úrbez-Torres">José Ram N Úrbez-Torres</name><affiliation wicri:level="1"><nlm:affiliation>Summerland Research and Development Centre, Agriculture and Agri-Food Canada, Summerland, BC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Summerland Research and Development Centre, Agriculture and Agri-Food Canada, Summerland, BC</wicri:regionArea><wicri:noRegion>BC</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Meifang" sort="Liu, Meifang" uniqKey="Liu M" first="Meifang" last="Liu">Meifang Liu</name><affiliation wicri:level="1"><nlm:affiliation>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author><author><name sortKey="Ayres, Matthew" sort="Ayres, Matthew" uniqKey="Ayres M" first="Matthew" last="Ayres">Matthew Ayres</name><affiliation wicri:level="1"><nlm:affiliation>South Australian Research and Development Institute, Adelaide, SA, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>South Australian Research and Development Institute, Adelaide, SA</wicri:regionArea><wicri:noRegion>SA</wicri:noRegion></affiliation></author><author><name sortKey="Sosnowski, Mark" sort="Sosnowski, Mark" uniqKey="Sosnowski M" first="Mark" last="Sosnowski">Mark Sosnowski</name><affiliation wicri:level="1"><nlm:affiliation>South Australian Research and Development Institute, Adelaide, SA, Australia; and School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Glen Osmond, SA, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>South Australian Research and Development Institute, Adelaide, SA, Australia; and School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Glen Osmond, SA</wicri:regionArea><wicri:noRegion>SA</wicri:noRegion></affiliation></author><author><name sortKey="Savocchia, Sandra" sort="Savocchia, Sandra" uniqKey="Savocchia S" first="Sandra" last="Savocchia">Sandra Savocchia</name><affiliation wicri:level="1"><nlm:affiliation>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (classification)</term><term>Ascomycota (genetics)</term><term>Ascomycota (physiology)</term><term>Australia (MeSH)</term><term>DNA Primers (genetics)</term><term>DNA, Fungal (genetics)</term><term>DNA, Fungal (isolation & purification)</term><term>Plant Diseases (microbiology)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Reproducibility of Results (MeSH)</term><term>Species Specificity (MeSH)</term><term>Spores, Fungal (genetics)</term><term>Spores, Fungal (physiology)</term><term>Vitis (growth & development)</term><term>Vitis (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term><term>ADN fongique (isolement et purification)</term><term>Amorces ADN (génétique)</term><term>Ascomycota (classification)</term><term>Ascomycota (génétique)</term><term>Ascomycota (physiologie)</term><term>Australie (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Reproductibilité des résultats (MeSH)</term><term>Réaction de polymérisation en chaine en temps réel (méthodes)</term><term>Spores fongiques (génétique)</term><term>Spores fongiques (physiologie)</term><term>Spécificité d'espèce (MeSH)</term><term>Vitis (croissance et développement)</term><term>Vitis (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term><term>DNA, Fungal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Fungal</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Australia</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Ascomycota</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ascomycota</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term><term>Amorces ADN</term><term>Ascomycota</term><term>Spores fongiques</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN fongique</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Réaction de polymérisation en chaine en temps réel</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Ascomycota</term><term>Spores fongiques</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Ascomycota</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Reproducibility of Results</term><term>Species Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Australie</term><term>Reproductibilité des résultats</term><term>Spécificité d'espèce</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Australie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Botryosphaeria dieback, caused by species of Botryosphaeriaceae, is an important grapevine trunk disease in Australia. Inocula produced by the pathogens are primarily dispersed by rain splash and wind and infect pruning wounds leading to cankers, dieback, and eventually death of vines. The objective of this study was to develop molecular tools to detect and quantify Botryosphaeriaceae inocula from the environment. These tools are essential for investigating spore dispersal patterns of Botryosphaeriaceae pathogens in Australian vineyards. DNA extraction protocols were evaluated and one modified protocol was found suitable for extracting Botryosphaeriaceae DNA from artificially and naturally inoculated Burkard volumetric spore sampler tapes. Multispecies primers and a hydrolysis probe for quantitative PCR (qPCR) were further developed to detect and quantify Botryosphaeriaceae inocula from environmental samples. Specificity tests showed that the multispecies primers were able to amplify the DNA of 10 Botryosphaeriaceae species (58 isolates) found in Australia while none of the 27 nontarget fungal species (90 isolates) tested were amplified. The qPCR assay was suitable for amplifying purified DNA, synthetic DNA fragments (gBlocks), and mixed DNA from spore trap tapes. The qPCR method developed in this study was shown to be rapid and sensitive in detecting Botryosphaeriaceae inocula from the environment using spore traps.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30673411</PMID><DateCompleted><Year>2019</Year><Month>02</Month><Day>28</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>102</Volume><Issue>8</Issue><PubDate><Year>2018</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>Molecular Methods to Detect and Quantify Botryosphaeriaceae Inocula Associated With Grapevine Dieback in Australia.</ArticleTitle><Pagination><MedlinePgn>1489-1499</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-11-17-1854-RE</ELocationID><Abstract><AbstractText>Botryosphaeria dieback, caused by species of Botryosphaeriaceae, is an important grapevine trunk disease in Australia. Inocula produced by the pathogens are primarily dispersed by rain splash and wind and infect pruning wounds leading to cankers, dieback, and eventually death of vines. The objective of this study was to develop molecular tools to detect and quantify Botryosphaeriaceae inocula from the environment. These tools are essential for investigating spore dispersal patterns of Botryosphaeriaceae pathogens in Australian vineyards. DNA extraction protocols were evaluated and one modified protocol was found suitable for extracting Botryosphaeriaceae DNA from artificially and naturally inoculated Burkard volumetric spore sampler tapes. Multispecies primers and a hydrolysis probe for quantitative PCR (qPCR) were further developed to detect and quantify Botryosphaeriaceae inocula from environmental samples. Specificity tests showed that the multispecies primers were able to amplify the DNA of 10 Botryosphaeriaceae species (58 isolates) found in Australia while none of the 27 nontarget fungal species (90 isolates) tested were amplified. The qPCR assay was suitable for amplifying purified DNA, synthetic DNA fragments (gBlocks), and mixed DNA from spore trap tapes. The qPCR method developed in this study was shown to be rapid and sensitive in detecting Botryosphaeriaceae inocula from the environment using spore traps.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Billones-Baaijens</LastName><ForeName>Regina</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Úrbez-Torres</LastName><ForeName>José Ramón</ForeName><Initials>JR</Initials><AffiliationInfo><Affiliation>Summerland Research and Development Centre, Agriculture and Agri-Food Canada, Summerland, BC, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Meifang</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ayres</LastName><ForeName>Matthew</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>South Australian Research and Development Institute, Adelaide, SA, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sosnowski</LastName><ForeName>Mark</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>South Australian Research and Development Institute, Adelaide, SA, Australia; and School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Glen Osmond, SA, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Savocchia</LastName><ForeName>Sandra</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>National Wine and Grape Industry Centre, School of Agricultural and Wine Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>05</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001315" MajorTopicYN="N" Type="Geographic">Australia</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027843" MajorTopicYN="N">Vitis</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>1</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>1</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30673411</ArticleId><ArticleId IdType="doi">10.1094/PDIS-11-17-1854-RE</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li><li>Canada</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Billones Baaijens, Regina" sort="Billones Baaijens, Regina" uniqKey="Billones Baaijens R" first="Regina" last="Billones-Baaijens">Regina Billones-Baaijens</name></noRegion><name sortKey="Ayres, Matthew" sort="Ayres, Matthew" uniqKey="Ayres M" first="Matthew" last="Ayres">Matthew Ayres</name><name sortKey="Liu, Meifang" sort="Liu, Meifang" uniqKey="Liu M" first="Meifang" last="Liu">Meifang Liu</name><name sortKey="Savocchia, Sandra" sort="Savocchia, Sandra" uniqKey="Savocchia S" first="Sandra" last="Savocchia">Sandra Savocchia</name><name sortKey="Sosnowski, Mark" sort="Sosnowski, Mark" uniqKey="Sosnowski M" first="Mark" last="Sosnowski">Mark Sosnowski</name></country><country name="Canada"><noRegion><name sortKey="Urbez Torres, Jose Ram N" sort="Urbez Torres, Jose Ram N" uniqKey="Urbez Torres J" first="José Ram N" last="Úrbez-Torres">José Ram N Úrbez-Torres</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/GrapevineDiseaseV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000242 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000242 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= GrapevineDiseaseV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30673411
|texte= Molecular Methods to Detect and Quantify Botryosphaeriaceae Inocula Associated With Grapevine Dieback in Australia.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30673411" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a GrapevineDiseaseV1
| This area was generated with Dilib version V0.6.37. |  |